D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E406Q

Expression Data

Yield (mg/mL): 4.699

Kinetic Assay Data

**slope of E406Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.20E-02	7.35E-02	6.65E-02
B	25.00	3.50E-02	3.30E-02	3.90E-02
C	8.33	1.75E-02	1.55E-02	1.40E-02
D	2.78	5.00E-03	5.00E-03	5.50E-03
E	0.93	2.00E-03	2.00E-03	2.00E-03
F	0.31	1.00E-03		1.00E-03
G	0.10	5.00E-04	5.00E-04	5.00E-04
H	0.03	5.00E-04	5.00E-04

Yield (mg/mL): 4.699 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/4/2024.
Data uploaded by dudleym and last updated on 2024-04-18 11:20:59.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.27E-01
B	25.00	7.06E-01	7.15E-01	7.19E-01
C	8.33	5.07E-01	4.72E-01	4.64E-01
D	2.78	3.04E-01	2.95E-01	2.69E-01
E	0.93	1.37E-01	1.12E-01	1.32E-01
F	0.31	4.30E-02	4.35E-02	4.30E-02
G	0.10	1.55E-02	1.55E-02	1.45E-02
H	0.03	1.48E-16	1.48E-16

Yield (mg/mL): 6.54 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/4/2024.
Data uploaded by dudleym and last updated on 2024-04-18 11:20:45.

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.