Raw Data for BglB Variant N220S

Expression Data

Yield (mg/mL): 2.815

Kinetic Assay Data

slope of N220S A_{420 nm} (s⁻¹)

		[S] (mm)	1	2	3
	A	75.00	8.35E-05		8.34E-05
	B	25.00	3.37E-05		3.37E-05
	C	8.33	1.68E-05	1.67E-05	1.67E-05
	D	2.78	1.67E-05	5.00E-05	3.33E-05
	E	0.93	1.67E-05	1.67E-05	1.67E-05
	F	0.31	1.67E-05		1.67E-05
	G	0.10	1.67E-05	1.67E-05	1.67E-05
	H	0.03	1.67E-05	1.67E-05	1.67E-05

Yield (mg/mL): 2.815     Dilution Factor:  100×

Download these data

Protein purified on 4/9/2024 and assayed on 4/11/2024.
Data uploaded by cbendickson and last updated on 2024-04-16 18:03:57.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of N220S A_{420 nm} (s⁻¹)

		T (°C)	1	2	3
	A	30.0	1.48E-04	3.40E-05	6.57E-05
	B	33.6	3.40E-05	3.34E-05
	C	37.3		3.33E-05	5.01E-05
	D	39.1	5.01E-05		4.99E-05
	E	40.9	5.09E-05	3.40E-05	6.57E-05
	F	44.5	3.34E-05		3.33E-05
	G	48.2		1.67E-05	1.50E-05
	H	50.0		3.33E-05	3.30E-05

Download these data

Protein purified on 4/9/2024 and assayed on 4/11/2024.
Data uploaded by cbendickson and last updated on 2024-04-16 16:03:13.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of Alabama, Huntsville. For inquiries, please contact Sharifa: <str0012@uah.edu>.