D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L219V

Expression Data

Yield (mg/mL): 1.379

Kinetic Assay Data

**slope of L219V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.24E-02	8.56E-02	8.94E-02
B	25.00	7.84E-02	8.36E-02	8.52E-02
C	8.33	6.67E-02	6.86E-02	6.83E-02
D	2.78	4.15E-02	3.76E-02	3.88E-02
E	0.93	1.92E-02	1.85E-02	1.80E-02
F	0.31	6.95E-03	6.70E-03	6.70E-03
G	0.10	2.60E-03	2.50E-03
H	0.03	1.50E-04	1.50E-04

Yield (mg/mL): 1.379 Dilution Factor: 100×

Protein purified on 3/27/2024 and assayed on 4/3/2024.
Data uploaded by srumjahn and last updated on 2024-04-15 13:14:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.18E-01	3.21E-01	3.23E-01
B	25.00	2.69E-01	2.94E-01	2.93E-01
C	8.33	2.85E-01	2.64E-01	2.78E-01
D	2.78	1.43E-01	1.31E-01	1.62E-01
E	0.93	5.81E-02	6.11E-02	6.88E-02
F	0.31	2.32E-02	2.17E-02	2.29E-02
G	0.10	1.01E-02	7.90E-03	8.10E-03
H	0.03	2.50E-04	1.00E-04	5.00E-05

Yield (mg/mL): 3.32 Dilution Factor: 100×

Protein purified on 3/27/2024 and assayed on 4/3/2024.
Data uploaded by srumjahn and last updated on 2024-04-15 12:57:25.

Thermostability Assay Data

**slope of L219V A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0
B	48.3	1.50E-04	1.50E-04
C	45.7	2.00E-04	2.50E-04	3.00E-04
D	42.4	1.30E-03	1.65E-03	1.40E-03
E	37.7	1.95E-02	1.94E-02	1.78E-02
F	33.6	4.27E-02	3.71E-02	3.66E-02
G	31.3	4.01E-02	4.40E-02	4.55E-02
H	30.0

Protein purified on 27-03-24 and assayed on 10-04-24.
Data uploaded by srumjahn and last updated on 2024-04-15 13:42:28.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.