Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.96E-01	3.17E-01	3.26E-01
	B	25.00	2.79E-01	3.25E-01	2.97E-01
	C	8.33	2.60E-01	2.58E-01	2.86E-01
	D	2.78	1.15E-01	1.44E-01	1.24E-01
	E	0.93	5.43E-02	6.28E-02	6.10E-02
	F	0.31	2.28E-02	2.63E-02	2.18E-02
	G	0.10	8.05E-03	9.45E-03	8.00E-03
	H	0.03	2.50E-04	2.50E-04	3.00E-04

Yield (mg/mL): 3.32     Dilution Factor:  100×

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Protein purified on 3/27/2024 and assayed on 4/3/2024.
Data uploaded by srumjahn and last updated on 2024-04-15 13:00:22.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0
	B	48.3
	C	45.7	7.50E-04	8.00E-04
	D	42.4	4.16E-02	5.27E-02	5.69E-02
	E	37.7	1.67E-01	1.81E-01	1.73E-01
	F	33.6	1.93E-01	2.16E-01	1.98E-01
	G	31.3	2.05E-01	2.02E-01	1.86E-01
	H	30.0	2.15E-01	1.97E-01	2.03E-01

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Protein purified on 27-03-24 and assayed on 10-04-24.
Data uploaded by srumjahn and last updated on 2024-04-15 13:28:52.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.