Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.18E-01	3.21E-01	3.23E-01
	B	25.00	2.69E-01	2.94E-01	2.93E-01
	C	8.33	2.85E-01	2.64E-01	2.78E-01
	D	2.78	1.43E-01	1.31E-01	1.62E-01
	E	0.93	5.81E-02	6.11E-02	6.88E-02
	F	0.31	2.32E-02	2.17E-02	2.29E-02
	G	0.10	1.01E-02	7.90E-03	8.10E-03
	H	0.03	2.50E-04	1.00E-04	5.00E-05

Yield (mg/mL): 3.32     Dilution Factor:  100×

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Protein purified on 3/27/2024 and assayed on 4/3/2024.
Data uploaded by srumjahn and last updated on 2024-04-15 12:57:25.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.00E-04	4.50E-04	3.00E-04
	B	48.3	3.00E-04	3.00E-04
	C	45.7	7.50E-04	1.30E-03
	D	42.4	4.88E-02	4.59E-02	5.90E-02
	E	37.7	2.02E-01	1.97E-01	1.76E-01
	F	33.6
	G	31.3	2.08E-01	2.15E-01	2.04E-01
	H	30.0	2.26E-01	2.19E-01	2.17E-01

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Protein purified on 27-03-24 and assayed on 10-04-24.
Data uploaded by srumjahn and last updated on 2024-04-15 13:34:23.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.