Raw Data for BglB Variant R246K

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of R246K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	3.06E-04
	B	48.7		4.32E-03	3.44E-03
	C	46.2	9.43E-04	1.83E-03	2.43E-03
	D	42.3	2.81E-03	1.97E-03	4.05E-03
	E	37.7	1.18E-02	1.05E-02	1.21E-02
	F	33.9	2.77E-02	2.85E-02	2.85E-02
	G	31.4	2.24E-02	2.76E-02	2.78E-02
	H	30.0	2.35E-02	2.36E-02	2.87E-02

Download these data

Protein purified on 4/5/2024 and assayed on 4/12/2024.
Data uploaded by mkulik and last updated on 2024-04-12 12:37:22.

Reference Data for WT

slope of R246K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.11E-03	2.68E-03	5.21E-03
	B	48.7	2.47E-03	2.66E-03
	C	46.2	3.72E-03		4.16E-03
	D	42.3	2.92E-02	3.06E-02	2.96E-02
	E	37.7	9.53E-02	9.60E-02	9.52E-02
	F	33.9	9.98E-02	9.73E-02	7.86E-02
	G	31.4	8.40E-02	7.76E-02	7.24E-02
	H	30.0	9.00E-02		8.90E-02

Download these data

Protein purified on 4/5/2024 and assayed on 4/12/2024.
Data uploaded by afeuer and last updated on 2024-04-12 12:37:27.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.