Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.92

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.4696	0.4383	0.3891
	B	25.00	0.3207	0.3229	0.3213
	C	8.33	0.2715	0.2753	0.2652
	D	2.78	0.09405	0.09495	0.0951
	E	0.93	0.0393	0.03795	0.0417
	F	0.31	0.0111	0.01425	0.01365
	G	0.10	0.0051	0.00525	0.00525
	H	0.03	0.0005998	0.0006	0.00075

Yield (mg/mL): 5.92     Dilution Factor:  100×

Download these data

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by luzsarrazola and last updated on 2024-04-12 11:54:25.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0006	0.0006	0.0006
	B	48.3	0.00075	0.0006	0.00045
	C	45.7		0.00105	0.00105
	D	42.4	0.01545	0.01575	0.0159
	E	37.7	0.141	0.1411	0.1223
	F	33.6	0.1557	0.1583	0.1587
	G	31.3	0.1451	0.1639	0.1491
	H	30.0	0.1203	0.1238

Download these data

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by luzsarrazola and last updated on 2024-04-12 12:01:18.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.