Raw Data for BglB Variant F397S

Expression Data

SDS-PAGE
 1.104

Kinetic Assay Data

slope of F397S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.02775 0.0267 0.02805
B 25.00 0.0234 0.0231 0.02325
C 8.33 0.01905 0.01905 0.0189
D 2.78 0.01035 0.01005
E 0.93 0.0042 0.00465 0.00435
F 0.31 0.00195 0.00195 0.0018
G 0.10 0.00105 0.00105 0.0009
H 0.03 0 0
 1.104      100×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by mariannegs and last updated on 2024-04-12 11:53:39.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4696 0.4383 0.3891
B 25.00 0.3207 0.3229 0.3213
C 8.33 0.2715 0.2753 0.2652
D 2.78 0.09405 0.09495 0.0951
E 0.93 0.0393 0.03795 0.0417
F 0.31 0.0111 0.01425 0.01365
G 0.10 0.0051 0.00525 0.00525
H 0.03 0.0005998 0.0006 0.00075
 5.92      100×

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by luzsarrazola and last updated on 2024-04-12 11:54:25.


Thermostability Assay Data

slope of F397S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00045
B 48.3 0.00075
C 45.7
D 42.4 0.0006 0.0006
E 37.7 0.0012 0.00105 0.0009
F 33.6 0.00165 0.00195 0.0015
G 31.3 0.00285 0.0024
H 30.0 0.00345 0.003

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by mariannegs and last updated on 2024-04-12 13:38:34.


Reference Data for WT

slope of F397S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006 0.0006 0.0006
B 48.3 0.00075 0.0006 0.00045
C 45.7 0.00105 0.00105
D 42.4 0.01545 0.01575 0.0159
E 37.7 0.141 0.1411 0.1223
F 33.6 0.1557 0.1583 0.1587
G 31.3 0.1451 0.1639 0.1491
H 30.0 0.1203 0.1238

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by luzsarrazola and last updated on 2024-04-12 12:01:18.


Design Data

7.613

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.