Raw Data for BglB Variant F397I

Expression Data

SDS-PAGE
 2.196

Kinetic Assay Data

slope of F397I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.42E-02 5.79E-02 5.51E-02
B 25.00 4.43E-02 4.49E-02 4.62E-02
C 8.33 3.84E-02 3.60E-02 3.69E-02
D 2.78 3.21E-02 2.73E-02 2.91E-02
E 0.93 1.91E-02 2.13E-02 1.77E-02
F 0.31 8.55E-03 7.65E-03
G 0.10 4.05E-03 4.65E-03 5.25E-03
H 0.03 6.00E-04 6.00E-04
 2.196      100×

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by AshleyKhouly and last updated on 2024-04-12 11:49:54.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.61E-01 4.48E-01 4.76E-01
B 25.00 3.57E-01 3.59E-01 3.85E-01
C 8.33 2.74E-01 2.63E-01 2.94E-01
D 2.78 1.60E-01 1.74E-01 1.74E-01
E 0.93 7.23E-02 7.49E-02 7.34E-02
F 0.31 2.67E-02 2.85E-02 2.87E-02
G 0.10 9.60E-03 9.75E-03 9.15E-03
H 0.03 0 0 0
 6.655      100×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-19 13:41:56.


Thermostability Assay Data

slope of F397I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 6.00E-04 6.00E-04 6.00E-04
C 45.7 0 0 0
D 42.4 1.05E-03 6.00E-04 7.50E-04
E 37.7 6.15E-03 6.00E-03 5.25E-03
F 33.6 1.14E-02 1.19E-02
G 31.3 1.29E-02 1.25E-02
H 30.0

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by AshleyKhouly and last updated on 2024-04-18 12:13:54.


Reference Data for WT

slope of F397I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04
B 48.3 7.50E-04 7.50E-04 7.50E-04
C 45.7 0 0 0
D 42.4 1.43E-02 1.43E-02
E 37.7 1.41E-01 1.55E-01 1.78E-01
F 33.6 1.98E-01 1.69E-01
G 31.3 1.52E-01 1.45E-01 1.67E-01
H 30.0 1.36E-01 1.58E-01 1.50E-01

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-12 12:07:41.


Design Data

26.92

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.