Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.178

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2023	0.2091	0.1929
	B	25.00	0.1698	0.1665	0.1743
	C	8.33	0.1572	0.1536	0.1513
	D	2.78	0.1227	0.1205	0.1233
	E	0.93	0.1045	0.099	0.0942
	F	0.31	0.0285	0.0393	0.06645
	G	0.10	0.01185	0.0129	0.01095
	H	0.03	0		0

Yield (mg/mL): 3.178     Dilution Factor:  100×

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Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by marcelaafanador and last updated on 2024-04-12 07:24:55.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	0
	B	48.3	0		0
	C	45.7	7.50E-04	6.00E-04	6.00E-04
	D	42.4	9.15E-03	1.01E-02
	E	37.7	9.41E-02	8.46E-02	8.13E-02
	F	33.6	1.05E-01	9.66E-02	8.72E-02
	G	31.3	1.05E-01	1.10E-01	9.33E-02
	H	30.0	1.05E-01	1.02E-01	1.04E-01

Download these data

Protein purified on 3/22/2024 and assayed on 4/29/2024.
Data uploaded by marcelaafanador and last updated on 2024-04-12 07:22:39.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.