Raw Data for BglB Variant E21D

Expression Data

SDS-PAGE
 0.4097

Kinetic Assay Data

slope of E21D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.90E-02 3.89E-02 4.14E-02
B 25.00 2.78E-02 2.94E-02 3.06E-02
C 8.33 2.45E-02 2.40E-02 2.49E-02
D 2.78 1.52E-02 1.59E-02 1.68E-02
E 0.93 7.35E-03 7.20E-03 7.80E-03
F 0.31 3.45E-03 3.45E-03 3.30E-03
G 0.10 1.65E-03 1.50E-03 1.65E-03
H 0.03 0 0
 0.4097      10×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by stephanyks and last updated on 2024-04-12 07:21:19.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.88E-01 2.81E-01 3.04E-01
B 25.00 3.07E-01 2.94E-01 3.06E-01
C 8.33 2.70E-01 2.74E-01 2.57E-01
D 2.78 2.93E-01 2.69E-01 2.65E-01
E 0.93 1.51E-01 1.45E-01 1.29E-01
F 0.31 4.97E-02 4.89E-02 5.37E-02
G 0.10 1.94E-02 2.07E-02
H 0.03 0 0
 5.999      100×

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by ivismesa and last updated on 2024-04-12 06:52:51.


Thermostability Assay Data

slope of E21D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 4.50E-04 4.50E-04
C 45.7 0 0 0
D 42.4 9.00E-04 1.05E-03 7.50E-04
E 37.7 4.35E-03 4.20E-03 3.60E-03
F 33.6 6.15E-03 5.85E-03 4.65E-03
G 31.3 7.20E-03 6.90E-03 6.15E-03
H 30.0 7.80E-03 7.65E-03 6.75E-03

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by stephanyks and last updated on 2024-04-12 07:31:20.


Reference Data for WT

slope of E21D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 7.50E-04 6.00E-04
C 45.7 7.50E-04 9.00E-04 1.05E-03
D 42.4 4.50E-04 6.00E-04
E 37.7 2.31E-01 2.25E-01 2.00E-01
F 33.6 2.01E-01 2.11E-01 2.02E-01
G 31.3 2.29E-01 2.30E-01 2.31E-01
H 30.0 2.41E-01 2.05E-01 2.10E-01

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by ivismesa and last updated on 2024-04-12 07:33:14.


Design Data

4.38

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.