Raw Data for Wild-Type BglB

Expression Data

No expression

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.22E-02		4.41E-02
	B	25.00	3.08E-02	3.26E-02	3.75E-02
	C	8.33	2.85E-02	3.09E-02	2.93E-02
	D	2.78	1.97E-02	1.80E-02	1.77E-02
	E	0.93	8.55E-03	8.70E-03	1.14E-02
	F	0.31	3.45E-03	4.35E-03	3.75E-03
	G	0.10	1.65E-03	1.95E-03	2.10E-03
	H	0.03	0	0	0

Yield (mg/mL): 0.7295     Dilution Factor:  100×

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Protein purified on 3/29/24 and assayed on 3/29/24.
Data uploaded by AMANDAC and last updated on 2024-05-09 11:16:53.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0
	B	48.3	0		0
	C	45.7	6.00E-04	7.50E-04	6.00E-04
	D	42.4	2.25E-03	2.10E-03
	E	37.7	7.95E-03	7.35E-03	7.95E-03
	F	33.6	1.40E-02	1.35E-02	1.37E-02
	G	31.3	1.23E-02	1.25E-02	1.07E-02
	H	30.0	1.19E-02	1.14E-02	1.19E-02

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Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by mayoral and last updated on 2024-05-09 11:20:50.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.