Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.0257

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.76E-01	2.90E-01	2.76E-01
	B	25.00	2.19E-01	2.25E-01	2.22E-01
	C	8.33	2.03E-01	1.96E-01	1.93E-01
	D	2.78	1.16E-01	1.18E-01	1.26E-01
	E	0.93	5.84E-02	5.35E-02	5.89E-02
	F	0.31	2.34E-02	2.51E-02	2.54E-02
	G	0.10	1.10E-02	1.13E-02	1.15E-02
	H	0.03	0	0	0

Yield (mg/mL): 1.0257     Dilution Factor:  100×

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Protein purified on 2/28/24 and assayed on 3/14/24.
Data uploaded by zizi197 and last updated on 2024-04-18 07:23:30.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	2.68E-01	2.43E-01
	B	30.5	2.24E-01	2.30E-01
	C	31.7	2.60E-01	2.47E-01
	D	33.2	0.22397	0.2219
	E	35.4	0.20503	0.23079
	F	38.3	0.24248	0.21724
	G	41.7	0.087103	0.097345
	H	44.5	0.0058966	0.0066207
	I	46.8	0	0.0028966
	J	48.3	0	0.0024828
	K	49.5	0	0
	L	50.0	0	0

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Protein purified on 2/28/24 and assayed on 3/21/24.
Data uploaded by zizi197 and last updated on 2024-04-11 07:12:47.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.