Raw Data for BglB Variant N270W

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of N270W A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.44E-03	2.51E-03	2.20E-03
	B	48.7	2.84E-03	2.57E-03	2.98E-03
	C	46.2	3.12E-03	3.59E-03	3.12E-03
	D	42.3	5.94E-03	5.79E-03
	E	37.7	4.99E-02	4.50E-02	4.94E-02
	F	33.9	7.22E-02	7.41E-02	7.44E-02
	G	31.4	7.07E-02	6.75E-02	7.25E-02
	H	30.0	7.32E-02	7.36E-02	7.47E-02

Download these data

Protein purified on 3/3/2024 and assayed on 3/10/2024.
Data uploaded by nmantha and last updated on 2024-04-10 12:46:38.

Reference Data for WT

slope of N270W A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	3.39E-03	4.60E-03	2.25E-03
	B	48.7	3.31E-03	2.94E-03	2.69E-03
	C	46.2	3.35E-03	5.43E-03	4.22E-03
	D	42.3	5.94E-02	6.43E-02	5.78E-02
	E	37.7	1.18E-01	1.18E-01	1.22E-01
	F	33.9	1.04E-01	1.22E-01	1.22E-01
	G	31.4	1.24E-01	1.24E-01	1.14E-01
	H	30.0	1.24E-01	1.15E-01	1.16E-01

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by fmeeker and last updated on 2024-04-10 12:56:40.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.