Raw Data for BglB Variant W325Q

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of W325Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00	0.00E+00
	B	48.7	0.00E+00	0.00E+00	0.00E+00
	C	46.2	0.00E+00	0.00E+00	0.00E+00
	D	42.3	5.10E-03	6.63E-03	5.67E-03
	E	37.7	1.86E-02	1.94E-02	2.13E-02
	F	33.9	2.10E-02	2.35E-02	2.37E-02
	G	31.4
	H	30.0	2.50E-02	2.53E-02	2.21E-02

Download these data

Protein purified on 4/2/24 and assayed on 4/9/24.
Data uploaded by mmalshagawi and last updated on 2024-04-09 12:55:25.

Reference Data for WT

slope of W325Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00	0.00E+00
	B	48.7	0.00E+00	0.00E+00	0.00E+00
	C	46.2		0.00E+00	0.00E+00
	D	42.3	5.48E-02	4.76E-02	4.69E-02
	E	37.7	7.53E-02	7.31E-02	7.42E-02
	F	33.9	8.07E-02	8.55E-02	7.80E-02
	G	31.4		8.74E-02	8.15E-02
	H	30.0	7.59E-02	8.57E-02	8.45E-02

Download these data

Protein purified on 4/2/2024 and assayed on 4/9/2024.
Data uploaded by cbenayed and last updated on 2024-04-09 12:40:33.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.