Raw Data for BglB Variant W325Q

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of W325Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00	0.00E+00
	B	48.7	0.00E+00	0.00E+00	0.00E+00
	C	46.2	0.00E+00	0.00E+00	0.00E+00
	D	42.3		4.43E-03	4.27E-03
	E	37.7	1.27E-02	1.40E-02	1.30E-02
	F	33.9	1.72E-02	1.60E-02	1.68E-02
	G	31.4	1.63E-02	1.56E-02	1.73E-02
	H	30.0	1.73E-02	1.75E-02	1.79E-02

Download these data

Protein purified on 4/2/2024 and assayed on 4/9/2024.
Data uploaded by GabasY and last updated on 2024-04-09 12:55:27.

Reference Data for WT

slope of W325Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00	0.00E+00
	B	48.7	0.00E+00	0.00E+00	0.00E+00
	C	46.2		0.00E+00	0.00E+00
	D	42.3	5.48E-02	4.76E-02	4.69E-02
	E	37.7	7.53E-02	7.31E-02	7.42E-02
	F	33.9	8.07E-02	8.55E-02	7.80E-02
	G	31.4		8.74E-02	8.15E-02
	H	30.0	7.59E-02	8.57E-02	8.45E-02

Download these data

Protein purified on 4/2/2024 and assayed on 4/9/2024.
Data uploaded by cbenayed and last updated on 2024-04-09 12:40:33.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.