D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F253I

Expression Data

Yield (mg/mL): 2.358

Kinetic Assay Data

**slope of F253I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.82E-01	2.79E-01	3.14E-01
B	25.00	2.35E-01	2.10E-01	2.61E-01
C	8.33	1.68E-01	1.81E-01	1.88E-01
D	2.78	8.77E-02	8.73E-02	9.72E-02
E	0.93	3.22E-02	3.37E-02	3.58E-02
F	0.31	1.34E-02	1.43E-02	1.38E-02
G	0.10	4.34E-03	5.19E-03	5.43E-03
H	0.03	1.36E-03	1.93E-03	1.66E-03

Yield (mg/mL): 2.358 Dilution Factor: 100×

Protein purified on 2/20/24 and assayed on 2/21/24.
Data uploaded by rtsimmon and last updated on 2024-04-02 14:36:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.61E-01	3.39E-01	2.65E-01
B	25.00	2.95E-01	2.89E-01	2.72E-01
C	8.33	2.04E-01	1.92E-01	2.00E-01
D	2.78	1.18E-01		1.04E-01
E	0.93	3.08E-02	3.42E-02	3.25E-02
F	0.31	1.51E-02	1.52E-02	1.35E-02
G	0.10	5.31E-03	4.90E-03	5.04E-03
H	0.03	1.71E-03	1.77E-03

Yield (mg/mL): 3.872 Dilution Factor: 100×

Protein purified on 2/20/24 and assayed on 2/21/24.
Data uploaded by rtsimmon and last updated on 2024-04-02 14:38:24.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.