D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325Q

Yield (mg/mL): 0.3572

**slope of W325Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.52E-02	7.00E-02	6.00E-02
B	25.00	7.34E-02	6.72E-02	6.44E-02
C	8.33	6.65E-02	6.02E-02	5.99E-02
D	2.78	5.02E-02	4.38E-02	4.61E-02
E	0.93	3.66E-02	3.11E-02	3.51E-02
F	0.31	2.73E-02	3.43E-02	3.27E-02
G	0.10	2.70E-02	2.54E-02	2.38E-02
H	0.03	1.92E-02	1.67E-02	1.69E-02

Yield (mg/mL): 0.3572 Dilution Factor: 10×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by hpacyon and last updated on 2024-03-26 11:13:38.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.06E-01	1.17E-01	1.14E-01
B	25.00	1.03E-01	1.04E-01	1.07E-01
C	8.33	7.46E-02	9.81E-02	9.26E-02
D	2.78	5.68E-02	6.80E-02	6.43E-02
E	0.93	4.20E-02	3.93E-02	4.25E-02
F	0.31	2.45E-02	2.47E-02	3.20E-02
G	0.10	2.16E-02	1.88E-02	2.56E-02
H	0.03	1.07E-02	1.53E-02	1.39E-02

Yield (mg/mL): 0.7292 Dilution Factor: 100×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by pmallari and last updated on 2024-03-26 10:33:09.

Rosetta Score for W325Q: 3.903

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.