D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315N

Yield (mg/mL): 0.6158

**slope of H315N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.00E-01	1.02E-01	1.12E-01
B	25.00	9.97E-02	9.17E-02	9.80E-02
C	8.33	9.02E-02	7.90E-02	1.03E-01
D	2.78	6.88E-02	6.22E-02	6.80E-02
E	0.93	4.65E-02	4.29E-02	4.59E-02
F	0.31	2.94E-02	3.63E-02	3.01E-02
G	0.10	3.02E-02	2.69E-02	2.53E-02
H	0.03	2.23E-02	2.35E-02	1.99E-02

Yield (mg/mL): 0.6158 Dilution Factor: 100×

Protein purified on 3/5/24 and assayed on 3/12/24.
Data uploaded by sazizuddin and last updated on 2024-03-25 17:17:23.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.38E-01	1.50E-01	1.46E-01
B	25.00	1.35E-01	1.45E-01	1.36E-01
C	8.33	1.02E-01	1.27E-01	1.29E-01
D	2.78	7.65E-02	9.62E-02	7.93E-02
E	0.93	5.72E-02	5.89E-02	5.50E-02
F	0.31	3.46E-02	3.17E-02	3.77E-02
G	0.10	2.96E-02	2.75E-02	2.52E-02
H	0.03	1.84E-02	2.00E-02	1.63E-02

Yield (mg/mL): 0.8753 Dilution Factor: 100×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by sprebus and last updated on 2024-03-26 11:35:28.

Rosetta Score for H315N: -1.52

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.