D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325Q

Yield (mg/mL): 0.2509

**slope of W325Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.06E-02	6.32E-02	6.16E-02
B	25.00	5.64E-02	5.97E-02	6.29E-02
C	8.33	5.02E-02	5.87E-02	5.01E-02
D	2.78	3.92E-02	5.03E-02	3.44E-02
E	0.93	2.86E-02	3.97E-02	3.10E-02
F	0.31	2.51E-02	2.79E-02	3.29E-02
G	0.10	2.53E-02	1.82E-02	2.90E-02
H	0.03	1.56E-02	1.56E-02	1.98E-02

Yield (mg/mL): 0.2509 Dilution Factor: 10×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by SMohammed and last updated on 2024-03-26 10:59:51.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.06E-01	1.17E-01	1.14E-01
B	25.00	1.03E-01	1.04E-01	1.07E-01
C	8.33	7.46E-02	9.81E-02	9.26E-02
D	2.78	5.68E-02	6.80E-02	6.43E-02
E	0.93	4.20E-02	3.93E-02	4.25E-02
F	0.31	2.45E-02	2.47E-02	3.20E-02
G	0.10	2.16E-02	1.88E-02	2.56E-02
H	0.03	1.07E-02	1.53E-02	1.39E-02

Yield (mg/mL): 0.7292 Dilution Factor: 100×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by pmallari and last updated on 2024-03-26 10:33:09.

Rosetta Score for W325Q: 3.903

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.