D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315N

Yield (mg/mL): 0.7745

**slope of H315N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.26E-01	1.32E-01	1.29E-01
B	25.00	1.27E-01	1.19E-01	1.25E-01
C	8.33	1.13E-01	1.12E-01	1.07E-01
D	2.78	8.22E-02	6.72E-02	8.08E-02
E	0.93	5.04E-02	4.28E-02	5.13E-02
F	0.31	3.21E-02	3.75E-02	3.43E-02
G	0.10	2.44E-02	2.54E-02	2.16E-02
H	0.03	1.59E-02	1.67E-02	1.54E-02

Yield (mg/mL): 0.7745 Dilution Factor: 100×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by Greena and last updated on 2024-03-15 17:08:28.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.38E-01	1.50E-01	1.46E-01
B	25.00	1.35E-01	1.45E-01	1.36E-01
C	8.33	1.02E-01	1.27E-01	1.29E-01
D	2.78	7.65E-02	9.62E-02	7.93E-02
E	0.93	5.72E-02	5.89E-02	5.50E-02
F	0.31	3.46E-02	3.17E-02	3.77E-02
G	0.10	2.96E-02	2.75E-02	2.52E-02
H	0.03	1.84E-02	2.00E-02	1.63E-02

Yield (mg/mL): 0.8753 Dilution Factor: 100×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by sprebus and last updated on 2024-03-26 11:35:28.

Rosetta Score for H315N: -1.52

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.