D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R246K

Yield (mg/mL): 0.5644

**slope of R246K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.38E-02	5.44E-02	6.03E-02
B	25.00	5.095E-02	4.58E-02	5.37E-02
C	8.33	3.785E-02	4.165E-02	3.99E-02
D	2.78	2.9E-02	3.375E-02
E	0.93	1.305E-02	1.735E-02	1.705E-02
F	0.31			8.45E-03
G	0.10			7.9E-03
H	0.03		7.25E-03

Yield (mg/mL): 0.5644 Dilution Factor: 100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by ableicher and last updated on 2024-03-15 12:53:37.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.15E-01	1.04E-01	9.96E-02
B	25.00	9.77E-02	8.85E-02	8.98E-02
C	8.33	6.73E-02	7.37E-02	7.24E-02
D	2.78	4.90E-02	5.82E-02	5.83E-02
E	0.93	2.19E-02		2.10E-02
F	0.31	1.01E-02	2.18E-02	1.53E-02
G	0.10	1.16E-02	5.50E-03	9.55E-03
H	0.03	3.85E-03	1.72E-02	7.40E-03

Yield (mg/mL): 1.091 Dilution Factor: 100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by afeuer and last updated on 2024-03-15 12:36:36.

Rosetta Score for R246K: -0.586

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.