D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N270W

Yield (mg/mL): 0.4437

**slope of N270W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.84E-02	3.80E-02	3.33E-02
B	25.00	2.42E-02	2.88E-02	2.80E-02
C	8.33	2.18E-02	2.34E-02	2.57E-02
D	2.78	1.61E-02	1.42E-02	1.56E-02
E	0.93	6.50E-03	6.50E-03	6.85E-03
F	0.31	2.80E-03	2.85E-03	2.80E-03
G	0.10	1.20E-03	1.40E-03	1.80E-03
H	0.03	3.80E-03	5.00E-04	2.15E-03

Yield (mg/mL): 0.4437 Dilution Factor: 100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by LLincoln and last updated on 2024-03-15 12:35:15.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.23E-02	9.64E-02	9.95E-02
B	25.00	7.94E-02	7.91E-02	7.78E-02
C	8.33	6.56E-02	6.62E-02	7.00E-02
D	2.78	4.34E-02	4.19E-02	4.39E-02
E	0.93	1.88E-02	2.14E-02	1.85E-02
F	0.31	7.80E-03	8.50E-03	8.60E-03
G	0.10		3.05E-03	3.40E-03
H	0.03	2.25E-03	1.45E-03

Yield (mg/mL): 1.053 Dilution Factor: 100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by cjahncke and last updated on 2024-03-15 12:35:36.

Rosetta Score for N270W: 8.565

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.