D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F243H

Yield (mg/mL): 0.8157

**slope of F243H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.54E-01	1.53E-01	1.48E-01
B	25.00	1.24E-01	1.16E-01	1.20E-01
C	8.33	9.21E-02	9.08E-02	9.77E-02
D	2.78	6.90E-02	6.21E-02	7.64E-02
E	0.93	6.12E-02		5.27E-02
F	0.31	4.18E-02	3.68E-02	4.07E-02
G	0.10	2.04E-02	2.89E-02	2.40E-02
H	0.03	1.84E-02	1.89E-02	1.57E-02

Yield (mg/mL): 0.8157 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by khawk and last updated on 2024-03-14 12:28:11.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.33E-01	1.45E-01	1.37E-01
B	25.00	1.16E-01	1.22E-01	1.28E-01
C	8.33	8.53E-02	1.15E-01	1.00E-01
D	2.78		8.20E-02	7.32E-02
E	0.93	3.59E-02		3.81E-02
F	0.31	2.12E-02	3.45E-02	2.80E-02
G	0.10	1.84E-02	1.69E-02
H	0.03	1.41E-02	1.45E-02	1.63E-02

Yield (mg/mL): 0.8935 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by sdube and last updated on 2024-03-14 12:27:51.

Rosetta Score for F243H: 2.79

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.