D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H379Y

Yield (mg/mL): 0.7524

**slope of H379Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.49E-02	5.89E-02	6.75E-02
B	25.00	5.19E-02	4.38E-02	5.54E-02
C	8.33		4.29E-02	5.31E-02
D	2.78	3.47E-02	2.96E-02
E	0.93
F	0.31
G	0.10		1.37E-02	1.37E-02
H	0.03	8.95E-03	9.85E-03

Yield (mg/mL): 0.7524 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by lgundersen and last updated on 2024-03-14 12:38:56.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.93E-02	9.87E-02	1.05E-01
B	25.00	7.53E-02	8.87E-02	9.44E-02
C	8.33	5.50E-02	6.94E-02	6.04E-02
D	2.78	3.67E-02	5.14E-02	4.23E-02
E	0.93	1.98E-02		2.12E-02
F	0.31	1.27E-02	1.66E-02	1.77E-02
G	0.10	9.55E-03	1.10E-02	1.21E-02
H	0.03	5.70E-03	6.80E-03	8.10E-03

Yield (mg/mL): 0.6486 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by ogallagher and last updated on 2024-03-28 11:13:14.

Rosetta Score for H379Y: -0.904

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.