D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R246K

Yield (mg/mL): 0.4653

**slope of R246K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.995E-02	9.130E-02	8.750E-02
B	25.00	8.220E-02	8.565E-02	8.920E-02
C	8.33	8.005E-02	6.845E-02	8.230E-02
D	2.78	6.545E-02	5.745E-02	6.460E-02
E	0.93	4.615E-02	3.875E-02	4.795E-02
F	0.31	4.490E-02	3.115E-02	4.120E-02
G	0.10	2.900E-02	2.830E-02	3.310E-02
H	0.03	2.440E-02	2.280E-02	2.070E-02

Yield (mg/mL): 0.4653 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by Slewis and last updated on 2024-03-13 12:41:33.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E-01	1.15E-01	1.18E-01
B	25.00	1.04E-01	1.14E-01	1.06E-01
C	8.33	7.14E-02	9.38E-02	9.75E-02
D	2.78	5.56E-02	7.15E-02	6.54E-02
E	0.93	4.07E-02	5.20E-02	4.04E-02
F	0.31	3.26E-02	2.88E-02	3.33E-02
G	0.10		2.82E-02	3.06E-02
H	0.03	1.64E-02	1.65E-02	2.10E-02

Yield (mg/mL): 0.6563 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by FNegri and last updated on 2024-03-13 12:48:50.

Rosetta Score for R246K: -0.586

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.