D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N270W

Yield (mg/mL): 0.5954

**slope of N270W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.63E-02	7.87E-02	8.77E-02
B	25.00	7.50E-02	6.97E-02	7.52E-02
C	8.33	7.29E-02	7.01E-02	6.99E-02
D	2.78	5.35E-02	4.99E-02	5.49E-02
E	0.93	3.02E-02	3.62E-02	3.05E-02
F	0.31	2.35E-02	2.84E-02	2.32E-02
G	0.10	1.99E-02	2.37E-02	2.19E-02
H	0.03	1.56E-02	1.54E-02	1.58E-02

Yield (mg/mL): 0.5954 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by rgarten and last updated on 2024-03-13 12:38:23.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.31E-01	1.27E-01	1.22E-01
B	25.00	1.04E-01	1.11E-01	1.05E-01
C	8.33	9.09E-02	1.00E-01	9.88E-02
D	2.78	6.78E-02	6.23E-02	6.13E-02
E	0.93	3.60E-02	3.36E-02	4.35E-02
F	0.31	2.58E-02	2.17E-02	2.76E-02
G	0.10	1.86E-02	1.79E-02	2.03E-02
H	0.03	1.25E-02	1.57E-02	1.42E-02

Yield (mg/mL): 1.064 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/13/2024.
Data uploaded by mparks and last updated on 2024-03-13 12:29:22.

Rosetta Score for N270W: 8.565

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.