D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315N

Yield (mg/mL): 1.021

**slope of H315N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.73E-02	1.10E-01	1.05E-01
B	25.00	1.01E-01	1.06E-01	1.00E-01
C	8.33	9.67E-02	9.52E-02	9.64E-02
D	2.78	6.49E-02	6.42E-02	7.15E-02
E	0.93	5.17E-02	4.81E-02	4.79E-02
F	0.31	3.01E-02	4.03E-02	3.41E-02
G	0.10	3.00E-02	2.69E-02	3.86E-02
H	0.03	1.91E-02	2.08E-02	2.55E-02

Yield (mg/mL): 1.021 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by cfremming and last updated on 2024-03-13 12:32:21.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.11E-01	1.35E-01	1.36E-01
B	25.00	1.15E-01	1.25E-01	1.18E-01
C	8.33	8.15E-02	1.07E-01	1.03E-01
D	2.78	6.65E-02	8.18E-02	6.38E-02
E	0.93	4.76E-02	4.42E-02	4.60E-02
F	0.31	2.79E-02	3.58E-02	3.28E-02
G	0.10	2.52E-02	2.87E-02	3.01E-02
H	0.03	1.15E-02	1.96E-02	1.50E-02

Yield (mg/mL): 0.8417 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by lbotelho and last updated on 2024-03-27 11:18:20.

Rosetta Score for H315N: -1.52

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.