D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E187F

Expression Data

Yield (mg/mL): 6.125

Kinetic Assay Data

**slope of E187F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.60E-01	7.07E-01	7.32E-01
B	25.00	5.94E-01	6.09E-01	6.01E-01
C	8.33	4.55E-01	4.74E-01	4.48E-01
D	2.78	2.67E-01	2.44E-01	2.63E-01
E	0.93	1.09E-01	1.07E-01	9.95E-02
F	0.31	3.85E-02	3.85E-02	3.75E-02
G	0.10	1.30E-02	1.30E-02	1.35E-02
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 6.125 Dilution Factor: 10×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by AsbelGonzalez and last updated on 2024-04-18 11:32:02.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6405		0.615
B	25.00	0.5205	0.493	0.543
C	8.33	0.439	0.441	0.396
D	2.78	0.2265	0.2275	0.237
E	0.93	0.099	0.1	0.1005
F	0.31	0.0405	0.035	0.037
G	0.10	0.012	0.013	0.013
H	0.03	1.48E-16	1.48E-16

Yield (mg/mL): 6.194 Dilution Factor: 100×

Protein purified on 4/4/24 and assayed on 4/11/24.
Data uploaded by cyazji and last updated on 2024-04-18 11:27:29.

Design Data

Rosetta Score for E187F: 1.822

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.