D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H182Y

Expression Data

Yield (mg/mL): 2.897

Kinetic Assay Data

**slope of H182Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	3.21E-01	3.88E-01	2.83E-01
C	8.33	2.43E-01	2.59E-01	2.69E-01
D	2.78	8.05E-02	8.35E-02	7.45E-02
E	0.93
F	0.31	3.65E-02	3.10E-02	3.20E-02
G	0.10	1.15E-02	1.15E-02	1.10E-02
H	0.03	0.00E+00		1.48E-16

Yield (mg/mL): 2.897 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by ArianaDawkins and last updated on 2024-04-18 11:20:57.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.93E-01	3.75E-01	5.45E-01
B	25.00	4.63E-01	5.73E-01	3.73E-01
C	8.33	3.60E-01	3.85E-01	3.30E-01
D	2.78	1.10E-01	1.27E-01	1.24E-01
E	0.93	1.27E-01	1.10E-01	1.14E-01
F	0.31		4.30E-02	3.80E-02
G	0.10		1.95E-02	1.85E-02
H	0.03		5.00E-04	5.00E-04

Yield (mg/mL): 4.779 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by PedroP and last updated on 2024-04-18 14:32:20.

Design Data

Rosetta Score for H182Y: 2.957

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.