D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T321S

Expression Data

Yield (mg/mL): 1.838

Kinetic Assay Data

**slope of T321S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.2407	0.1962
B	25.00		0.1548	0.1594
C	8.33	0.05925	0.08405	0.1118
D	2.78		0.07555	0.08395
E	0.93	0.03345	0.05825	0.0423
F	0.31	0.0384	0.0375	0.02825
G	0.10		0.01575	0.0117
H	0.03	0.00035	0.00025	0.0005

Yield (mg/mL): 1.838 Dilution Factor: 100×

Protein purified on 1/23/24 and assayed on 1/23/24.
Data uploaded by enniferj and last updated on 2024-03-27 23:08:23.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.3694	0.3505
B	25.00		0.2409	0.2354
C	8.33	0.1146	0.2249	0.1913
D	2.78	0.1066	0.1344	0.1738
E	0.93	0.08875	0.1004	0.075
F	0.31	0.06365	0.07885	0.0643
G	0.10	0.03455	0.0292	0.02515
H	0.03	0.00035	0.0003	0.00035

Yield (mg/mL): 2.684 Dilution Factor: 100×

Protein purified on 1/23/24 and assayed on 1/23/24.
Data uploaded by enniferj and last updated on 2024-03-27 19:11:20.

Design Data

Rosetta Score for T321S: 2.413

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.