D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant C167S

Expression Data

Yield (mg/mL): 2.278

Kinetic Assay Data

**slope of C167S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.81E-02	5.36E-02	5.59E-02
B	25.00	2.71E-02	2.64E-02	2.65E-02
C	8.33	1.03E-02	9.55E-03	1.09E-02
D	2.78	3.70E-03	3.85E-03	3.85E-03
E	0.93	1.30E-03	1.45E-03	1.40E-03
F	0.31	8.50E-04	6.00E-04	6.50E-04
G	0.10	3.50E-04	6.00E-04	4.50E-04
H	0.03	0	3.50E-04	1.40E-03

Yield (mg/mL): 2.278 Dilution Factor: 10×

Protein purified on 11/15/23 and assayed on 11/30/23.
Data uploaded by mvlouie and last updated on 2023-12-07 16:36:47.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.09E-01	3.13E-01	3.09E-01
B	25.00	2.49E-01	2.41E-01	2.37E-01
C	8.33	1.79E-01	1.74E-01	1.55E-01
D	2.78	9.94E-02	1.01E-01	9.95E-02
E	0.93	4.55E-02	4.53E-02	4.42E-02
F	0.31	1.70E-02	1.65E-02	1.66E-02
G	0.10	6.10E-03	5.90E-03	5.75E-03
H	0.03	2.35E-03	2.10E-03	2.20E-03

Yield (mg/mL): 3.678 Dilution Factor: 100×

Protein purified on 11/15/23 and assayed on 11/17/23.
Data uploaded by mvlouie and last updated on 2023-11-28 12:17:38.

Design Data

Rosetta Score for C167S: 2.973

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.