D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M114S

Expression Data

Yield (mg/mL): 1.176

Kinetic Assay Data

**slope of M114S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.05E-02	7.57E-02	7.78E-02
B	25.00	5.20E-02	5.49E-02	5.38E-02
C	8.33	3.44E-02	3.52E-02	3.40E-02
D	2.78	1.75E-02	1.71E-02	1.88E-02
E	0.93	7.45E-03	8.00E-03	7.05E-03
F	0.31	2.85E-03	2.45E-03	2.75E-03
G	0.10	1.05E-03	1.00E-03
H	0.03	5.50E-04	4.50E-04	5.00E-04

Yield (mg/mL): 1.176 Dilution Factor: 100×

Protein purified on 11/15/23 and assayed on 11/30/23.
Data uploaded by mvlouie and last updated on 2023-12-07 15:10:26.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.09E-01	3.13E-01	3.09E-01
B	25.00	2.49E-01	2.41E-01	2.37E-01
C	8.33	1.79E-01	1.74E-01	1.55E-01
D	2.78	9.94E-02	1.01E-01	9.95E-02
E	0.93	4.55E-02	4.53E-02	4.42E-02
F	0.31	1.70E-02	1.65E-02	1.66E-02
G	0.10	6.10E-03	5.90E-03	5.75E-03
H	0.03	2.35E-03	2.10E-03	2.20E-03

Yield (mg/mL): 3.678 Dilution Factor: 100×

Protein purified on 11/15/23 and assayed on 11/17/23.
Data uploaded by mvlouie and last updated on 2023-11-28 12:17:38.

Design Data

Rosetta Score for M114S: 5.021

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.