Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.491

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.87E-01	3.66E-01	3.68E-01
	B	25.00	2.69E-01	2.80E-01	2.75E-01
	C	8.33
	D	2.78		1.43E-01	1.38E-01
	E	0.93	6.53E-02	6.48E-02	6.30E-02
	F	0.31	2.34E-02	2.32E-02	2.32E-02
	G	0.10	8.60E-03	8.10E-03	9.50E-03
	H	0.03	4.00E-04	3.50E-04

Yield (mg/mL): 1.491     Dilution Factor:  100×

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Protein purified on 11/9/23 and assayed on 11/16/23.
Data uploaded by Leopreciado and last updated on 2023-12-07 13:02:18.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.00E+00	0.00E+00
	B	48.3	2.50E-04		3.00E-04
	C	45.7	6.00E-04		5.00E-04
	D	42.4	4.17E-02	4.49E-02
	E	37.7	2.52E-01	2.47E-01	2.39E-01
	F	33.6	2.47E-01	2.35E-01	2.46E-01
	G	31.3	2.71E-01	2.82E-01	2.65E-01
	H	30.0	2.69E-01	2.65E-01	2.66E-01

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Protein purified on 11/9/2023 and assayed on 11/18/2023.
Data uploaded by suziebailey and last updated on 2023-12-20 08:22:08.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.