Raw Data for BglB Variant E406D

Expression Data

SDS-PAGE
 0.486

Kinetic Assay Data

slope of E406D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.70E-03 2.75E-03 2.80E-03
B 25.00 1.50E-03 1.60E-03 1.55E-03
C 8.33 9.00E-04 9.50E-04 1.00E-03
D 2.78 6.00E-04 5.50E-04 5.50E-04
E 0.93 4.50E-04 3.50E-04 4.00E-04
F 0.31 1.50E-04 2.00E-04 3.00E-04
G 0.10 1.50E-04 0 3.50E-04
H 0.03 2.50E-04 3.00E-04
 0.486      100×

Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by Roseline and last updated on 2023-12-20 08:48:35.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3191 0.318 0.3013
B 25.00 0.2246 0.2261 0.22
C 8.33 0.1997 0.1651 0.172
D 2.78 0.1281 0.1511 0.1249
E 0.93 0.0561 0.0573 0.05295
F 0.31 0.0215 0.0214 0.02035
G 0.10 0.0073 0.0074 0.0063
H 0.03 0.00015 0 0.0002
 1.441      100×

Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by AndreaskZImmermann and last updated on 2023-12-07 12:51:03.


Thermostability Assay Data

slope of E406D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 0 7.00E-04
B 48.3 2.50E-04 0 1.50E-04
C 45.7 5.50E-04 1.50E-04 2.50E-04
D 42.4 1.45E-03 1.10E-03 9.50E-04
E 37.7 7.40E-03 7.30E-03 7.65E-03
F 33.6 7.75E-03 7.95E-03 8.65E-03
G 31.3 7.90E-03 8.00E-03 8.20E-03
H 30.0 8.15E-03 8.30E-03 8.20E-03

Protein purified on 11/9/2023 and assayed on 11/18/2023.
Data uploaded by JoeBreslin and last updated on 2023-12-07 12:40:37.


Reference Data for WT

slope of E406D A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.2612 0.2489 0.253
B 31.3 0.2489 0.2491 0.2561
C 33.6 0.2481 0.2463 0.2515
D 37.7 0.2406 0.2416 0.2405
E 42.4 0.0583 0.04825 0.04055
F 45.7 0.0222 0.0205
G 48.3 0.00935 0.0002 0.0164
H 50.0 0.00085 0.0002 0.0018

Protein purified on 11/9/2023 and assayed on 11/18/2023.
Data uploaded by AndreaskZImmermann and last updated on 2023-12-07 12:34:54.


Design Data

-4.441

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.