Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.2

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2902	0.4261	0.6031
	B	25.00	0.4913	0.4497	0.4381
	C	8.33	0.2737		0.2509
	D	2.78	0.1612	0.1424	0.1593
	E	0.93	0.08130		0.07510
	F	0.31		0.02380	0.02690
	G	0.10		0.005900	0.004450
	H	0.03	0.007550	0.005700	0.006400

Yield (mg/mL): 1.2     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/29/2023.
Data uploaded by Madhavi and last updated on 2023-12-06 18:00:23.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0		0
	B	48.3	0.0007000	0.0005999
	C	45.7		0.001200	0.001100
	D	42.4	0.05770	0.06070
	E	37.7	0.2902	0.3082	0.2903
	F	33.6	0.3040	0.3138
	G	31.3	0.0006000	0.0006000
	H	30.0	0.0007000		0.0006000

Download these data

Protein purified on 11/8/2023 and assayed on 11/29/2023.
Data uploaded by Madhavi and last updated on 2023-12-06 18:01:39.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.