D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A80P

Expression Data

No expression

Kinetic Assay Data

**slope of A80P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.00035	0.00055	0.00080
B	25.00	0.00050	0.00040	0.00045
C	8.33	0.00035	0.00035
D	2.78	0.00035	0.0004	0.00030
E	0.93	0.00035	0.00025	0.00030
F	0.31	0.00025	0.00025	0.00025
G	0.10	0.00025	0.00025	0.00025
H	0.03	0.00025	0.00025	0.00030

Yield (mg/mL): 0.3 Dilution Factor: 10×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by DanielLevin and last updated on 2023-12-01 21:36:37.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.07E-01	1.08E-01
B	25.00	8.61E-02	9.66E-02	9.07E-02
C	8.33	6.95E-02	8.12E-02	5.77E-02
D	2.78	4.94E-02	4.82E-02	6.31E-02
E	0.93	3.82E-02	3.29E-02	4.33E-02
F	0.31	3.14E-02	2.84E-02
G	0.10	2.75E-02	2.53E-02
H	0.03	2.84E-02	2.85E-02	3.82E-02

Yield (mg/mL): 1.7 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:46:06.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of A80P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	5.00E-04	4.50E-04	4.00E-04
B	48.3	3.50E-04	3.50E-04	3.00E-04
C	45.7	7.00E-04		7.00E-04
D	42.4	2.76E-02	2.72E-02
E	37.7	1.02E-01	1.13E-01	1.18E-01
F	33.6	1.37E-01	1.26E-01	1.46E-01
G	31.3	1.41E-01	1.27E-01	1.44E-01
H	30.0	1.13E-01	1.22E-01	1.13E-01

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:54:30.

Design Data

Rosetta Score for A80P: 190.938

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.