D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W247V

Expression Data

Yield (mg/mL): 0.16

Kinetic Assay Data

Plate 20201217-alex

**slope of W247V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.000973333	0.000984242	0.000764242
B	18.75	0.000547273	0.000543636	0.000378182
C	4.69	0.000410909	0.000386667
D	1.17	0.000334545	0.000224242	0.000141818
E	0.29	0.000164242	0.000119394	5.27E-05
F	0.07		3.82E-05	0
G	0.02	4.91E-05		3.94E-05
H	0.00	0.000030303		2.42E-05

Yield / A_{280 nm}: 0.16 Dilution Factor: 10×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0118679	0.0116933	0.0100139
B	18.75	7.23E-03	0.00763152	6.20E-03
C	4.69	5.29E-03	5.77E-03
D	1.17	4.11E-03	4.31E-03	3.40E-03
E	0.29		1.59E-03	1.62E-03
F	0.07	4.50E-04	4.82E-04
G	0.02	2.56E-04	1.78E-04	3.31E-04
H	0.00	1.15E-04		1.30E-04

Yield / A_{280 nm}: 0.275 Dilution Factor: 100×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.