Raw Data for BglB Variant P277M

Expression Data

SDS-PAGE
 0.448

Kinetic Assay Data

slope of P277M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.31E-02 1.22E-02 1.10E-02
B 25.00 1.06E-02 9.40E-03 8.80E-03
C 8.33 8.40E-03 7.70E-03 7.30E-03
D 2.78 6.50E-03 5.60E-03 5.20E-03
E 0.93 4.20E-03 3.40E-03 3.90E-03
F 0.31 3.10E-03 2.70E-03 2.50E-03
G 0.10 2.20E-03 1.70E-03 1.50E-03
H 0.03 2.00E-04 2.00E-04
 0.448      100×

Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by rakowbg23 and last updated on 2023-11-30 11:06:25.


Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.



Thermostability Assay Data

Data missing or deleted from the database.


Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.



Design Data

4.495


Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.