D2D CURE | Data | Raw Characterization Data

Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.041

Kinetic Assay Data

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.18E-01	2.41E-01	2.30E-01
B	25.00	1.72E-01	1.83E-01	1.84E-01
C	8.33	1.52E-01	1.59E-01	1.61E-01
D	2.78	9.78E-02	1.13E-01	9.99E-02
E	0.93	4.92E-02	5.99E-02	5.15E-02
F	0.31	2.21E-02	2.72E-02	2.54E-02
G	0.10	8.70E-03	1.26E-02	1.07E-02
H	0.03	1.65E-03	1.65E-03	1.65E-03

Yield (mg/mL): 5.041 Dilution Factor: 100×

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicoFuentes98 and last updated on 2023-11-29 22:15:52.

Thermostability Assay Data

**slope of WT A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0		0
B	48.3	1.05E-03	6.00E-04	0
C	45.7	1.05E-03	6.00E-04	7.50E-04
D	42.4	8.55E-03	8.55E-03
E	37.7	9.38E-02	1.03E-01	7.55E-02
F	35.6	1.12E-01	1.12E-01	8.67E-02
G	31.3	1.11E-01	1.11E-01	8.85E-02
H	30.0	1.13E-01	1.02E-01

Protein purified on 10/30/23 and assayed on 11/6/23.
Data uploaded by NicoFuentes98 and last updated on 2023-11-30 06:27:15.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.