D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y144W

Expression Data

Yield (mg/mL): 1.702

Kinetic Assay Data

**slope of Y144W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1490	0.1500	0.1560
B	25.00	0.1200	0.1180	0.1170
C	8.33	0.0990	0.0853	0.0985
D	2.78	0.1100	0.0768	0.0819
E	0.93	0.02820	0.03520	0.03720
F	0.31		0.02100	0.01890
G	0.10		0.007500	0.007150
H	0.03	0.000400	0.000250

Yield (mg/mL): 1.702 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/14/2023.
Data uploaded by Ixchel and last updated on 2023-12-28 17:22:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1280	0.1270	0.1260
B	25.00	0.1190	0.1030	0.1070
C	8.33	0.1050	0.0854	0.0831
D	2.78	0.0499		0.0467
E	0.93	0.02050	0.02830	0.02340
F	0.31	0.01100	0.01400
G	0.10	0.003050	0.005500	0.000450
H	0.03	0.000500	0.000450

Yield (mg/mL): 1.7 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/14/2023.
Data uploaded by Ixchel and last updated on 2024-01-17 15:40:10.

Design Data

Rosetta Score for Y144W: -8.794

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.