D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant C167H

Expression Data

Yield (mg/mL): 0.358

Kinetic Assay Data

Plate 20201217-tali2

**slope of C167H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.00886909	0.00823455	0.00648848
B	18.75	0.00589758	0.00740788	0.00637576
C	4.69		0.00457636	0.0043497
D	1.17		0.00231273	0.00220242
E	0.29	0.00024	0.000462424	0.000593333
F	0.07	9.09E-05	2.38E-04	1.61E-04
G	0.02	3.58E-05	4.73E-05
H	0.00	2.91E-05	3.15E-05	4.06E-05

Yield / A_{280 nm}: 0.358 Dilution Factor: 100×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0102655	0.00766848
B	18.75	0.00849818	0.00768848
C	4.69	0.0049703	0.00447758
D	1.17	0.00229455	0.00184606
E	0.29	0.000653939	0.000593939
F	0.07	0.000184242	0.000178788
G	0.02	1.37E-04		0.000159394
H	0.00	1.70E-05		2.30E-05

Yield / A_{280 nm}: 0.102 Dilution Factor: 50×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.