Raw Data for BglB Variant H328K

Expression Data

SDS-PAGE
 1.6

Kinetic Assay Data

slope of H328K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0554 0.0574
B 25.00
C 8.33 0.0507 0.0571 0.0563
D 2.78 0.0355 0.0376 0.0353
E 0.93 0.02545 0.02795 0.02410
F 0.31 0.01845 0.01885 0.01990
G 0.10 0.01475 0.01515 0.01605
H 0.03 0.015500 0.014700 0.015650
 1.6      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by JaeheeLee and last updated on 2023-11-29 15:09:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.07E-01 1.08E-01
B 25.00 8.61E-02 9.66E-02 9.07E-02
C 8.33 6.95E-02 8.12E-02 5.77E-02
D 2.78 4.94E-02 4.82E-02 6.31E-02
E 0.93 3.82E-02 3.29E-02 4.33E-02
F 0.31 3.14E-02 2.84E-02
G 0.10 2.75E-02 2.53E-02
H 0.03 2.84E-02 2.85E-02 3.82E-02
 1.7      100×

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:46:06.


Thermostability Assay Data

slope of H328K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0003500 0.000350
C 45.7 0.000500 0.000400 0.000450
D 42.4 0.00685 0.00605 0.00645
E 37.7 0.0479 0.0550 0.0481
F 33.6 0.0553 0.0575 0.0608
G 31.3 0.0764 0.0647 0.0778
H 30.0 0.0683 0.0668 0.0661

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by JaeheeLee and last updated on 2023-11-29 15:13:14.


Reference Data for WT

slope of H328K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 4.50E-04 4.00E-04
B 48.3 3.50E-04 3.50E-04 3.00E-04
C 45.7 7.00E-04 7.00E-04
D 42.4 2.76E-02 2.72E-02
E 37.7 1.02E-01 1.13E-01 1.18E-01
F 33.6 1.37E-01 1.26E-01 1.46E-01
G 31.3 1.41E-01 1.27E-01 1.44E-01
H 30.0 1.13E-01 1.22E-01 1.13E-01

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:54:30.


Design Data

1.096

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.