D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R240A

Expression Data

Yield (mg/mL): 3.3

Kinetic Assay Data

**slope of R240A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6283	0.3915	0.7599
B	25.00	0.2035	0.2348
C	8.33	0.1503	0.1684
D	2.78		0.1696	0.1555
E	0.93	0.04840	0.07610	0.06255
F	0.31	0.01655	0.01710	0.01520
G	0.10	0.00665	0.01045	0.00285
H	0.03	0		0

Yield (mg/mL): 3.3 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by Ryan and last updated on 2023-11-29 15:12:47.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0696	0.0785	0.0693
B	25.00	0.0523	0.0540	0.0563
C	8.33	0.0550	0.0555	0.0512
D	2.78	0.0389	0.0376	0.0368
E	0.93	0.02680	0.02435	0.01735
F	0.31	0.00890	0.01100	0.00830
G	0.10	0.00385		0.00345
H	0.03	0.000200	0	0.000300

Yield (mg/mL): 0.9 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by manyarohloff and last updated on 2023-11-29 15:11:33.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of R240A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.00040	0.0004000
B	48.3	0.0006000	0.000400	0.0005000
C	45.7	0.001100	0.000900	0.001000
D	42.4	0.02560	0.02820	0.03290
E	37.7	0.0770	0.0776
F	33.6	0.1330	0.1150	0.1180
G	31.3
H	30.0

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by manyarohloff and last updated on 2023-12-21 18:39:22.

Design Data

Rosetta Score for R240A: 2.307

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.