Raw Data for BglB Variant E340A

Expression Data

SDS-PAGE
 2.7

Kinetic Assay Data

slope of E340A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1685 0.1532 0.1704
B 25.00 0.1703 0.2215
C 8.33 0.1530 0.1807
D 2.78 0.1080 0.1188 0.1049
E 0.93 0.07020 0.10110 0.10150
F 0.31 0.06195 0.07410 0.07430
G 0.10 0.05890 0.06180 0.06420
H 0.03 0.065600 0.054950 0.063650
 2.7      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by AndriaYu and last updated on 2023-11-29 14:56:16.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.07E-01 1.08E-01
B 25.00 8.61E-02 9.66E-02 9.07E-02
C 8.33 6.95E-02 8.12E-02 5.77E-02
D 2.78 4.94E-02 4.82E-02 6.31E-02
E 0.93 3.82E-02 3.29E-02 4.33E-02
F 0.31 3.14E-02 2.84E-02
G 0.10 2.75E-02 2.53E-02
H 0.03 2.84E-02 2.85E-02 3.82E-02
 1.7      100×

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:46:06.


Thermostability Assay Data

slope of E340A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0003000 0.000350
B 48.3
C 45.7 0.000500 0.000500
D 42.4 0.00835 0.01000 0.00960
E 37.7 0.1561 0.1597 0.1502
F 33.6 0.2025 0.2204 0.2445
G 31.3 0.2401 0.2478 0.2409
H 30.0 0.2394 0.2443 0.2226

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by AndriaYu and last updated on 2023-11-29 15:02:38.


Reference Data for WT

slope of E340A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 4.50E-04 4.00E-04
B 48.3 3.50E-04 3.50E-04 3.00E-04
C 45.7 7.00E-04 7.00E-04
D 42.4 2.76E-02 2.72E-02
E 37.7 1.02E-01 1.13E-01 1.18E-01
F 33.6 1.37E-01 1.26E-01 1.46E-01
G 31.3 1.41E-01 1.27E-01 1.44E-01
H 30.0 1.13E-01 1.22E-01 1.13E-01

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:54:30.


Design Data

-17.583

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.