Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.7

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		1.07E-01	1.08E-01
	B	25.00	8.61E-02	9.66E-02	9.07E-02
	C	8.33	6.95E-02	8.12E-02	5.77E-02
	D	2.78	4.94E-02	4.82E-02	6.31E-02
	E	0.93	3.82E-02	3.29E-02	4.33E-02
	F	0.31	3.14E-02	2.84E-02
	G	0.10	2.75E-02	2.53E-02
	H	0.03	2.84E-02	2.85E-02	3.82E-02

Yield (mg/mL): 1.7     Dilution Factor:  100×

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Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:46:06.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	5.00E-04	4.50E-04	4.00E-04
	B	48.3	3.50E-04	3.50E-04	3.00E-04
	C	45.7	7.00E-04		7.00E-04
	D	42.4	2.76E-02	2.72E-02
	E	37.7	1.02E-01	1.13E-01	1.18E-01
	F	33.6	1.37E-01	1.26E-01	1.46E-01
	G	31.3	1.41E-01	1.27E-01	1.44E-01
	H	30.0	1.13E-01	1.22E-01	1.13E-01

Download these data

Protein purified on and assayed on .
Data uploaded by laylasemaan and last updated on 2023-11-29 14:54:30.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.