Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.4

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1618	0.1632	0.1467
	B	25.00	0.1251	0.1295	0.1422
	C	8.33	0.09470	0.08805	0.09315
	D	2.78	0.07140	0.06960	0.07660
	E	0.93	0.03465	0.03455	0.04030
	F	0.31	0.01480	0.01450	0.01650
	G	0.10	0.005650	0.005600	0.006700
	H	0.03	0.002450	0.002000	0.002450

Yield (mg/mL): 1.4     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by joshhyun and last updated on 2023-11-29 11:59:56.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.0006000	0.0005000
	B	48.3	0.001000	0.0007001	0.0006000
	C	45.7	0.001900	0.001800
	D	42.4	0.06880	0.06880	0.06040
	E	37.7	0.1368	0.1501	0.1249
	F	33.6	0.1500	0.1335	0.1107
	G	31.3	0.1380	0.1077	0.1246
	H	30.0	0.1141	0.1182	0.1101

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by joshhyun and last updated on 2023-11-29 12:05:16.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.