Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.5

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.14E-01	3.22E-01	3.12E-01
	B	25.00	2.81E-01	2.81E-01	2.80E-01
	C	8.33	2.43E-01	2.13E-01	2.25E-01
	D	2.78	1.50E-01	1.40E-01	1.46E-01
	E	0.93	6.52E-02	6.18E-02	6.41E-02
	F	0.31	2.95E-02	2.73E-02	3.32E-02
	G	0.10	1.35E-02	1.24E-02	1.29E-02
	H	0.03	4.65E-03	4.50E-03	5.00E-03

Yield (mg/mL): 2.5     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by TaylonB and last updated on 2023-11-29 11:59:50.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.44E-02	6.00E-04	5.60E-03
	B	48.3	6.00E-04		5.00E-04
	C	45.7	1.30E-03	1.00E-03	1.50E-03
	D	42.4		4.78E-02	4.94E-02
	E	37.7	1.80E-01	1.82E-01	1.69E-01
	F	33.6	2.11E-01	2.00E-01	2.06E-01
	G	31.3	2.13E-01	2.18E-01	2.15E-01
	H	30.0	1.56E-01	1.67E-01	1.65E-01

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by TaylonB and last updated on 2023-11-29 12:04:53.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.