Raw Data for BglB Variant H328K

Expression Data

SDS-PAGE
 1.7

Kinetic Assay Data

slope of H328K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.62E-01 2.55E-01
B 25.00 2.10E-01 8.44E-02 1.76E-01
C 8.33 1.38E-01 1.07E-01 9.07E-02
D 2.78 7.53E-02 7.42E-02
E 0.93 1.83E-02 2.75E-02 2.08E-02
F 0.31 9.80E-03 1.00E-02 9.95E-03
G 0.10 3.90E-03 2.95E-03 2.35E-03
H 0.03 3.75E-03 2.80E-03 2.65E-03
 1.7      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by elise and last updated on 2023-11-29 12:01:13.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2026 0.2552 0.3866
B 25.00 0.2959 0.3224 0.2882
C 8.33 0.2211 0.2181 0.2233
D 2.78 0.1429 0.1312 0.1342
E 0.93 0.06365 0.06025 0.0582
F 0.31 0.01305 0.0193 0.01585
G 0.10 0.0116 0.0089 0.00905
H 0.03 0.00855 0.0106 0.0077
 1.4      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by rrachel11 and last updated on 2023-11-29 11:59:31.


Thermostability Assay Data

slope of H328K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 6.00E-04 0 4.00E-04
C 45.7 6.00E-04 7.00E-04
D 42.4 9.00E-03 1.60E-02 3.34E-02
E 37.7 1.49E-01 1.46E-01 1.84E-01
F 33.6 2.03E-01 1.93E-01 2.10E-01
G 31.3 2.27E-01 1.95E-01 1.78E-01
H 30.0 2.11E-01 2.06E-01 1.67E-01

Protein purified on 11/8/23 and assayed on 11/15/23.
Data uploaded by elise and last updated on 2023-11-29 12:18:25.


Reference Data for WT

slope of H328K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0007 0.0005
C 45.7 0.0008 0.0011 0.001
D 42.4 0.0217 0.0212 0.0252
E 37.7 0.1001 0.0943
F 33.6 0.0961 0.0947
G 31.3 0.1006 0.0981 0.0941
H 30.0 0.0902 0.0968 0.0784

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by rrachel11 and last updated on 2023-11-29 12:02:38.


Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.