Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.4

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2026	0.2552	0.3866
	B	25.00	0.2959	0.3224	0.2882
	C	8.33	0.2211	0.2181	0.2233
	D	2.78	0.1429	0.1312	0.1342
	E	0.93	0.06365	0.06025	0.0582
	F	0.31	0.01305	0.0193	0.01585
	G	0.10	0.0116	0.0089	0.00905
	H	0.03	0.00855	0.0106	0.0077

Yield (mg/mL): 1.4     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by rrachel11 and last updated on 2023-11-29 11:59:31.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	0
	B	48.3		0.0007	0.0005
	C	45.7	0.0008	0.0011	0.001
	D	42.4	0.0217	0.0212	0.0252
	E	37.7		0.1001	0.0943
	F	33.6		0.0961	0.0947
	G	31.3	0.1006	0.0981	0.0941
	H	30.0	0.0902	0.0968	0.0784

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by rrachel11 and last updated on 2023-11-29 12:02:38.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.